Protein Mass Spectrometry Sample Prep
Waters Corporation GlycoWorks RapiFluor-MS N-Glycan Starter Kit, 96 Sample, HILIC, MS/MS, UV, HRMS Detection
This starter kit offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans. It simplifies sample preparation to three easy steps, achievable in as little as 30 minutes. The RapiFluor-MS reagent provides increased fluorescence quantification and mass spectral response, enabling valuable information from characterization to routine monitoring for 96 samples.
- Streamlined, fast, and highly sensitive N-glycan analysis.
- Three easy sample preparation steps in as little as 30 minutes.
- RapiFluor-MS reagent for increased fluorescence quantification and mass spectral response.
- Processes 96 samples in a 4x24 format.
- Includes modules for deglycosylation, labeling, clean-up, and sample collection.
- Optimized for glycoprotein quantity up to 40 μg.
- Rapid workflow: deglycosylation <15 min, labeling 5 min, clean-up 10 min.
Waters Corp Waters N-Glycan Kit, RapiFluor-MS, 96 Sample
This kit provides a streamlined, fast, and highly sensitive method for analyzing released N-glycans. It simplifies complicated and time-consuming sample preparation into three easy steps that can be completed in as little as 30 minutes. The RapiFluor-MS label offers increased fluorescence quantification and superior mass spectral response, allowing for a single label to provide valuable information from characterization to routine monitoring. This core kit contains materials to process 96 samples in a 4x24 format.
- Streamlined, fast, and highly sensitive analysis of released N-glycans.
- Sample preparation in as little as 30 minutes through 3 easy steps.
- Label provides increased fluorescence quantification and superior mass spectral response.
- Utilizes a single label for characterization and routine monitoring.
- Processes 96 samples in a 4x24 format.
Waters Corp Waters GlycoWorks RapiFluor-MS N-Glycan Basic Kit, 96 Sample
This basic kit is designed to streamline and simplify N-glycan sample preparation. It allows for analysis in three easy steps, taking as little as 30 minutes. The RapiFluor-MS technology provides increased fluorescence quantification and enhanced mass spectral response, enabling the use of a single label for characterization to routine monitoring.
- Simplifies and speeds up N-glycan sample preparation to just 3 easy steps in as little as 30 minutes.
- Utilizes RapiFluor-MS technology for enhanced fluorescence quantification and mass spectral response.
- Allows for sensitive glycan analysis and comprehensive characterization using a single label.
- Processes 96 samples in a 4x24 format.
- Reduces complicated, time-consuming sample preparation.
- Enables easier and more sensitive glycan analysis.
- Provides in-depth characterization of low abundance N-glycans.
Waters Corporation N-Glycan Kit, RapiFluor-MS, 24 Sample, Sample Preparation Workflow
The GlycoWorks RapiFluor-MS N-Glycan Kit by Waters Corporation offers a fast, sensitive method for N-glycan analysis. It streamlines sample preparation to three steps in approximately 30 minutes, utilizing RapiFluor-MS for enhanced fluorescence and mass spectral response. This kit is validated for monoclonal antibodies and other N-linked glycoproteins.
- Optimized for 24 analyses and a 3-step sample preparation workflow (deglycosylation, labeling, purification) in ≈30 minutes.
- Utilizes HILIC separation mode and supports MS, MS/MS, UV, HRMS detection with LC-MS systems for glycan applications.
- Optimized for 15 to 40 μg glycoprotein per reaction.
- Features RapiFluor-MS label for sensitive fluorescence detection and strong mass detection, and HILIC μElution Plate SPE for clean-up.
Waters Corp N-Glycan Script Starter Kit, Automated, 96 Analysis, HILIC
The GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit-Automation is designed for high-throughput glycoprotein sample preparation and N-glycan analysis using automated platforms. This kit enables minimal user intervention and is validated with monoclonal antibodies and various N-linked glycoproteins.
- Designed for automated platforms
- Enables high-throughput glycoprotein sample preparation
- Supports N-glycan analysis
- Requires minimal user intervention
- Protocol validated using monoclonal antibodies
- Tested for a wide range of other N-linked glycoproteins
- Reagents scaled for automated instrumentation versatility
- Kit provided in a 2 x 48 format
- Includes CD and Workstation requirements document with script
- Includes RFMS labeled standard, column, and mobile phase concentrate for RapiFluor-MS Library use
Waters Corp ProteinWorks eXpress Digest Start-up Kit, 96 Analyses, Protein Quantification, LC-MS
This kit simplifies and accelerates direct digestion of immunopurified plasma and serum samples for LC-MS protein quantification. It includes pre-measured, lot-traceable reagents, a 96-tube sample collection module, and optimized protocols for accurate protein quantification.
- Accelerates direct digestion of immunopurified plasma and serum samples for LC-MS protein quantification.
- Contains pre-measured, lot-traceable reagents and optimized protocols.
- Offers a 96-tube sample collection module for 96 analyses.
- Delivers single-digit intra-kit and inter-kit reproducibility for monoclonal antibodies.
- Increases assay sensitivity and system resilience by removing excess digest reagents and plasma/serum components.
- Ensures laboratory and data consistency via a validated, standardized technique.
Waters Corp N-Glycan Eco Starter Kit, Automation, RapiFluor-MS, 2 x 48 Format
The GlycoWorks RapiFluor-MS N-Glycan Eco Starter Kit – Automation is designed for automated platforms, enabling high-throughput glycoprotein sample preparation for N-glycan analysis. It is validated for monoclonal antibodies and other N-linked glycoproteins. This kit uses DMSO as a greener, safer alternative for the RapiFluor-MS Reagent Label, replacing DMF.
- Designed for automated glycoprotein sample preparation.
- Validated for monoclonal antibodies and N-linked glycoproteins.
- Reagents scaled for automated instrumentation.
- Replaces DMF with greener DMSO for RapiFluor-MS Reagent Label.
- Removes other DMF-containing solutions.
- Includes RFMS labeled standard, column, and mobile phase concentrate.
- Kit format: 2 x 48.
- Suggested use: Eco-friendly, released N-linked glycan analysis, automation.
Waters Corp N-Glycan Sample Preparation Kit, Eco-Friendly, Automation, 2 x 48 Format
This kit enables high-throughput glycoprotein sample preparation for N-glycan analysis, ideal for automated platforms with minimal user intervention. Validated with monoclonal antibodies and effective for various N-linked glycoproteins, this eco-friendly kit replaces DMF with DMSO and removes other DMF-containing solutions. It is provided in a 2 x 48 format.
- Rapid labeling for sensitive fluorescence detection and strong mass signal intensity.
- Streamlined protocol with fast deglycosylation, labeling, and cleanup steps.
- Designed for full automation on compatible platforms, processing 96 samples in under 3 hours.
- Optimized reagents ensure efficient deglycosylation and prevent precipitation.
- Efficient cleanup through solid-phase extraction removes reaction by-products.
Waters Corp ProteinWorks eXpress Digest Kit - Ambient Refill Kit
ProteinWorks eXpress Digest Kit - Ambient Refill Kit
Biotium Spastin(Sp 6C6), CF488A conjugate, 0.1mg/mL
The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40% of all autosomal dominant HSPs. Primary antibodies are available purified, or